Lilemong tsa morao tjena, li-biobanks li ntse li bapala karolo ea bohlokoa le ho feta lipatlisisong tsa mahlale.Thepa ea boleng bo holimo ea boleng bo tlase ba polokelo e ka netefatsa polokeho le tšebetso ea lisampole le ho thusa bafuputsi ho etsa liphuputso tse fapaneng tsa mahlale hamolemo ka ho fana ka tikoloho e bolokehileng le e bolokehileng ea polokelo bakeng sa disampole tsa baeloji.
Litanka tsa naetrojene tse metsi li 'nile tsa sebelisoa ho boloka lisampole nako e telele.Ba boloka lisampole ka mocheso o tlase oa -196 ℃ o entsoeng ho latela molao-motheo oa ho kenya vacuum ka mor'a hore lisampole li pholile pele.Ho na le mekhoa e 'meli bakeng sa litanka tsa naetrojene tse metsi ho boloka lisampole: polokelo ea mohato oa mokelikeli le polokelo ea mouoane.Phapano ke efe pakeng tsa tse peli?
1. Kopo
Litanka tsa naetrojene tsa mokhahlelo oa metsi li sebelisoa haholo lilaboratoring, leruong la liphoofolo, le lefapheng la ho lokisa.
Litanka tsa "Vapor phase liquid nitrogen" li sebelisoa haholo libankang tsa bioloji, meriana le lebaleng la tlhokomelo ea bophelo.
2. Boemo ba polokelo
Mokhahlelong oa mouoane, lisampole li bolokoa ka ho fetoha mouoane le ho pholisa metsi a naetrojene.Mocheso oa mocheso oa polokelo o tloha holimo ho ea tlaase sebakeng sa polokelo ea mohlala.Ha ho bapisoa, mohatong oa mokelikeli, lisampole li bolokoa ka kotloloho ho naetrojene e metsi ho -196 ° C.Mehlala e lokela ho qoelisoa ka ho feletseng ka har'a metsi a naetrojene.
Haier Biomedical Liquid Nitrogen Container-Smart Series
Ho phaella phapanong ena, litekanyetso tsa mouoane oa naetrojene ea metsi tse peli le tsona li fapane.Ka kakaretso, sekhahla sa mouoane oa naetrojene ea metsi se ipapisitse le bophara ba tanka ea naetrojene e mokelikeli, makhetlo a basebelisi ba bulang sekoaelo, mokhoa oa tlhahiso, esita le mocheso le mongobo o teng.Empa ka tlhaho, mahlale a tsoetseng pele a vacuum le a insulation a sebelisoang ho etsa litanka tsa naetrojene tsa metsi ke senotlolo sa ho netefatsa tšebeliso e tlase ea naetrojene ea metsi.
Phapang e kholo ka ho fetisisa pakeng tsa tse peli e itšetlehile ka tsela eo lisampole li bolokiloeng ka eona.E bolokiloe mokhahlelong oa mouoane, lisampole ha li kopane ka kotloloho le naetrojene ea metsi, ho thibela libaktheria ho silafatsa lisampole.Leha ho le joalo, mocheso oa polokelo ha o khone ho fihla ho -196°C.Karolong ea mokelikeli, le hoja lisampole li ka bolokoa ho pota -196 ° C, "cryopreservation tube" ha e tsitsa.Haeba tube ea cryopreservation e sa koaloa hantle, naetrojene e metsi e tla kenella ka har'a tube.Ha tube ea teko e ntšoa, ho feto-fetoha ha naetrojene ea metsi ho tla lebisa khatellong e sa leka-lekaneng ka hare le ka ntle ho tube ea teko 'me tube e tla phatloha ka lebaka leo.Ka hona, botšepehi ba sampuli bo tla lahleha.Sena se fana ka maikutlo a hore ho na le melemo le boiketlo ho mokhoa o mong le o mong.
Joang ho otla ho leka-lekana pakeng tsa tse peli?
Letoto la biobank la Haier Biomedical Liquid Nitrogen Storage System e etselitsoe polokelo ea mokelikeli le mouoane.
E kopanya melemo ea polokelo ea mohato oa mouoane le polokelo ea mokelikeli, e entsoeng ka theknoloji e tsoetseng pele ea vacuum le ho kenya letsoho ho netefatsa polokeho ea polokelo le ho tšoana ha mocheso ha e ntse e fokotsa tšebeliso ea metsi a nitrogen.Phapang ea mocheso oa sebaka sohle sa polokelo ha e fete 10°C.Esita le mokhahlelong oa mouoane, mocheso oa polokelo o haufi le holim'a shelofo o tlase ho -190°C.
Letoto la Biobank Bakeng sa polokelo e kholo ea Scale
Ho feta moo, ho sebelisoa li-sensor tsa mocheso o nepahetseng haholo le boemo ba mokelikeli ho netefatsa ho nepahala.Lintlha tsohle le lisampole li sirelelitsoe ke mokhoa o sireletsehileng oa taolo ea phihlello.Li-sensor tsena li lekola lintlha tsa mocheso le boemo ba mokelikeli ka har'a tanka ea naetrojene ea metsi ka nako ea 'nete, ka hona metsi a ka tankeng a ka tlatsoa hape ho theha maemo a bolokehileng a polokelo ea sampole.
Nako ea poso: Feb-26-2024
